Mapping of DNA-binding proteins along the yeast genome by UV-induced DNA-protein crosslinking

D A Papatsenko; I V Priporova; S V Belikov; V L Karpov

doi:10.1016/0014-5793(96)00091-9

Mapping of DNA-binding proteins along the yeast genome by UV-induced DNA-protein crosslinking

FEBS Lett. 1996 Feb 26;381(1-2):103-5. doi: 10.1016/0014-5793(96)00091-9.

Authors

D A Papatsenko¹, I V Priporova, S V Belikov, V L Karpov

Affiliation

¹ W.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.

PMID: 8641413
DOI: 10.1016/0014-5793(96)00091-9

Abstract

UV-induced crosslinking of DNA-binding proteins to DNA in intact nuclei of Saccharomyces cerevisiae and subsequent 'protein image' hybridization were applied to map non-histone proteins along single-copy genes of yeast. We detected two polypeptides that most probably correspond to core subunits of yeast RNA-polymerase II in the coding region of transketolase gene (TKL2). Several non-histone proteins were also detected which bind to the upstream region of TKL2 gene, and to the intergenic spacer between calmodulin (CMD1) and beta-mannosyl transferase (ALG1) genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Calmodulin / genetics
DNA, Fungal / analysis
DNA, Fungal / metabolism*
DNA, Fungal / radiation effects
DNA-Binding Proteins / analysis
DNA-Binding Proteins / metabolism*
DNA-Binding Proteins / radiation effects
Genes, Fungal*
Genome, Fungal*
Introns
Mannosyltransferases / genetics
RNA Polymerase II / analysis
RNA Polymerase II / metabolism
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / metabolism*
Ultraviolet Rays*

Substances

Calmodulin
DNA, Fungal
DNA-Binding Proteins
Mannosyltransferases
chitobiosyldiphosphodolichol beta-mannosyltransferase
RNA Polymerase II