Assignment of the human alpha-tropomyosin gene TPM4 to band 19p13.1 by fluorescence in situ hybridization

S D Wilton; L Lim; S D Dorosz; H C Gunn; H J Eyre; D F Callen; N G Laing

doi:10.1159/000134206

Assignment of the human alpha-tropomyosin gene TPM4 to band 19p13.1 by fluorescence in situ hybridization

Cytogenet Cell Genet. 1996;72(4):294-6. doi: 10.1159/000134206.

Authors

S D Wilton¹, L Lim, S D Dorosz, H C Gunn, H J Eyre, D F Callen, N G Laing

Affiliation

¹ Australian Neuromuscular Research Institute, QE II Medical Centre, Nedlands, Western Australia, Australia.

PMID: 8641132
DOI: 10.1159/000134206

Abstract

Sequence-tagged sites (STSs) were developed for the human alpha-tropomyosin gene TPM4. One STS was used to amplify DNA from somatic cell hybrids to localize TPM4 to chromosome 19. The other, a product from a long-range PCR, was used directly as a probe to refine the localization of TPM4 to 19p13.1 by fluorescence in situ hybridization to metaphase chromosome spreads.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 19 / genetics*
DNA Primers / genetics
DNA Probes / genetics
Humans
Hybrid Cells
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Tagged Sites
Tropomyosin / genetics*

Substances

DNA Primers
DNA Probes
Tropomyosin