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Dev Biol. 1996 Feb 25;174(1):115-24.

Analysis of regulatory elements of the developmentally controlled chorion s15 promoter in transgenic Drosophila.

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  • 1Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107-5099, USA.

Abstract

The Drosophila s15 chorion gene is expressed only in the follicular epithelium surrounding the developing oocyte, with tight quantitative control and a very narrow temporal specificity. We have used germ-line transformation analysis to conduct an extensive mutational dissection of its promoter between -189 and -39 bp relative to the transcriptional start site. Quantitative control and temporal specificity are disrupted by several of these mutations. The results suggest that this 150-bp DNA region encompasses many positive and negative, at least partially degenerate, cis-regulatory elements, which are involved in specifying the highly precise expression pattern of the s15 gene during development.

PMID:
8626011
[PubMed - indexed for MEDLINE]
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