Display Settings:

Format

Send to:

Choose Destination
Fertil Steril. 1996 May;65(5):1067-70.

A new method to study the process of implantation of a human blastocyst in vitro.

Author information

  • 1Department of Woman and Child Health, Karolinska Hospital, Stockholm, Sweden.

Abstract

OBJECTIVES:

To develop a "miniorgan" culture of human endometrium dated according to the LH peak in order to study the process of implantation of a human blastocyst in vitro.

DESIGN:

Preliminary results of a more extensive study with the same objectives.

SETTING:

Hospital-based unit of reproductive health and university-related reproductive research laboratories.

PATIENTS:

Six apparently healthy women with normal regular menstrual cycles providing endometrial material 4, 5 and 6 days after the LH peak.

INTERVENTION:

One part of the biopsy was used for histologic dating. The other part was incubated in culture medium RPMI-1640 and exposed to the contact with one to three embryos fertilized in vitro.

MAIN OUTCOME MEASURE:

Biopsy material before and after incubation was studied by morphometric methods in a light microscope. The histologic changes were recorded by photomicrographs.

RESULTS:

Implantation of an embryo obtained 4 days after IVF penetrated the lining endometrial epithelium of a biopsy obtained 4 days after the LH peak and kept in vitro for 24 hours. The embryo was placed on the lining epithelium of the endometrium within 3 hours after the biopsy was taken.

CONCLUSION:

A new method to study the process of implantation of a human embryo in vitro has been described, allowing the embryo to penetrate the lining endometrial epithelium in a biopsy obtained at LH +4/+5.

PMID:
8612839
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk