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Science. 1996 Mar 22;271(5256):1690-2.
Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns.
Department of Molecular Biology, Cleveland Clinic Foundation, OH 44195, USA.
A conserved sequence element in a minor class of eukaryotic pre-messenger RNA (pre-mRNA) introns was previously proposed to base pair with a complementary sequence in the U12 small nuclear RNA (snRNA) in a manner analogous to the pairing of US snRNA with the branch site sequence of the major class of introns. Here, mutations generated in this conserved sequence element block the splicing of a member of this minor intron class in vivo. The block was relieved by coexpression of a U12 snRNA containing compensatory mutations that restore the proposed base pairing interaction. These results show that this minor class of pre-mRNA introns is a distinct class existing alongside the major class of introns in animal genomes, and these results also establish an in vivo function for U12 snRNA.
PMID: 8596930 [PubMed - indexed for MEDLINE]
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Cited by 55 PubMed Central articles
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Mutational analysis of the U12-dependent branch site consensus sequence.
Brock JE, Dietrich RC, Padgett RA.
RNA. 2008 Nov; 14(11):2430-9. Epub 2008 Sep 29.
[RNA. 2008]
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Minor-class splicing occurs in the nucleus of the Xenopus oocyte.
Friend K, Kolev NG, Shu MD, Steitz JA.
RNA. 2008 Aug; 14(8):1459-62. Epub 2008 Jun 20.
[RNA. 2008]
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Where in the cell is the minor spliceosome?
Steitz JA, Dreyfuss G, Krainer AR, Lamond AI, Matera AG, Padgett RA.
Proc Natl Acad Sci U S A. 2008 Jun 24; 105(25):8485-6. Epub 2008 Jun 17.
[Proc Natl Acad Sci U S A. 2008]
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