Role of a truncated erythropoietin receptor for erythroid differentiation

Biochem Biophys Res Commun. 1996 Jan 5;218(1):205-9. doi: 10.1006/bbrc.1996.0036.

Abstract

Erythropoietin (EPO) is a cytokine that regulates erythropoiesis through the EPO receptor (EPOR). We reported previously that erythroid progenitor cells express both a full-length and a truncated form of EPOR (EPOR-F and EPOR-Tph). EPOR-Tph cannot transmit growth signals and acts as a dominant negative regulator against EPOR-F-mediated signals for cell survival and growth. Upon transfection of EPOR-F in a cell line, Ba/F3, beta-globin accumulation, which is considered to be a marker of erythroid-differentiation, is inducible in the transformants. We show here that the co-expression of EPOR-Tph in EPOR-F-transformants does not inhibit and rather upregulates the beta-globin induction while inhibiting survival and growth of the transformants. These data suggest that, in contrast to survival and growth signals, EPOR-Tph acts as a positive regulator for erythroid-differentiation signals in erythroid progenitor cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Differentiation* / drug effects
  • Cell Division
  • Cell Line
  • Cell Survival
  • DNA Primers
  • Erythropoietin / pharmacology*
  • Globins / biosynthesis
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / metabolism
  • Kinetics
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / biosynthesis
  • Receptors, Erythropoietin / biosynthesis*
  • Receptors, Erythropoietin / physiology
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / metabolism
  • Signal Transduction
  • Transfection

Substances

  • DNA Primers
  • RNA, Messenger
  • Receptors, Erythropoietin
  • Recombinant Proteins
  • Erythropoietin
  • Globins