Cancer Res. 1996 Jan 15;56(2):235-40.

Expression of the human mismatch repair gene hMSH2 in normal and neoplastic tissues.

Leach FS, Polyak K, Burrell M, Johnson KA, Hill D, Dunlop MG, Wyllie AH, Peltomaki P, de la Chapelle A, Hamilton SR, Kinzler KW, Vogelstein B.

Source

Johns Hopkins Oncology Center, Baltimore, Maryland 21231, USA.

Abstract

Hereditary nonpolyposis colorectal cancer is caused by inherited mutations of mismatch repair genes. We developed monoclonal antibodies to the prototype human mismatch repair gene hMSH2 and used them to detect an immunoreactive protein of M(r) 100,000 in mismatch-proficient cell lines. In addition, a M(r) 150,000 protein coimmunoprecipitated with the hMSH2 gene product in cell lines expressing hMSH2. Immunohistochemistry demonstrated that the hMSH2 protein was exclusively nuclear. Whereas the hMSH2 protein was expressed in a variety of tissues, the most striking pattern was observed in esophageal and intestinal epithelia, where expression was limited to the replicating compartment. Neoplastic cells within benign and malignant mismatch repair-proficient tumors expressed the protein, but no hMSH2 immunoreactivity was observed in the colorectal tumors of patients with germline hMSH2 mutation. These results have implications for tumorigenic mechanisms and, potentially, for diagnosis.

PMID:: 8542572; [PubMed - indexed for MEDLINE]

Free full text

LinkOut - more resources

Supplemental Content

Save items

Related citations in PubMed

Altered expression of hMSH2 and hMLH1 in tumors with microsatellite instability and genetic alterations in mismatch repair genes. [Cancer Res. 1996]
Altered expression of hMSH2 and hMLH1 in tumors with microsatellite instability and genetic alterations in mismatch repair genes.
Thibodeau SN, French AJ, Roche PC, Cunningham JM, Tester DJ, Lindor NM, Moslein G, Baker SM, Liskay RM, Burgart LJ, et al. Cancer Res. 1996 Nov 1; 56(21):4836-40.
Clinical features and mismatch repair gene mutation screening in Chinese patients with hereditary nonpolyposis colorectal carcinoma. [World J Gastroenterol. 2004]
Clinical features and mismatch repair gene mutation screening in Chinese patients with hereditary nonpolyposis colorectal carcinoma.
Liu SR, Zhao B, Wang ZJ, Wan YL, Huang YT. World J Gastroenterol. 2004 Sep 15; 10(18):2647-51.
Immunohistochemistry for hMLH1 and hMSH2: a practical test for DNA mismatch repair-deficient tumors. [Am J Surg Pathol. 1999]
Immunohistochemistry for hMLH1 and hMSH2: a practical test for DNA mismatch repair-deficient tumors.
Marcus VA, Madlensky L, Gryfe R, Kim H, So K, Millar A, Temple LK, Hsieh E, Hiruki T, Narod S, et al. Am J Surg Pathol. 1999 Oct; 23(10):1248-55.
Mutational analysis of promoters of mismatch repair genes hMSH2 and hMLH1 in hereditary nonpolyposis colorectal cancer and early onset colorectal cancer patients: identification of three novel germ-line mutations in promoter of the hMSH2 gene. [Cancer Res. 2002]
Mutational analysis of promoters of mismatch repair genes hMSH2 and hMLH1 in hereditary nonpolyposis colorectal cancer and early onset colorectal cancer patients: identification of three novel germ-line mutations in promoter of the hMSH2 gene.
Shin KH, Shin JH, Kim JH, Park JG. Cancer Res. 2002 Jan 1; 62(1):38-42.
Review Molecular diagnostics of cancer predisposition: hereditary non-polyposis colorectal carcinoma and mismatch repair defects. [Biochim Biophys Acta. 1999]
Review Molecular diagnostics of cancer predisposition: hereditary non-polyposis colorectal carcinoma and mismatch repair defects.
Bocker T, Rüschoff J, Fishel R. Biochim Biophys Acta. 1999 May 31; 1423(3):O1-O10.

See reviews... See all...

Cited by 17 PubMed Central articles

Immunohistochemistry versus microsatellite instability testing for screening colorectal cancer patients at risk for hereditary nonpolyposis colorectal cancer syndrome. Part I. The utility of immunohistochemistry. [J Mol Diagn. 2008]
Immunohistochemistry versus microsatellite instability testing for screening colorectal cancer patients at risk for hereditary nonpolyposis colorectal cancer syndrome. Part I. The utility of immunohistochemistry.
Shia J. J Mol Diagn. 2008 Jul; 10(4):293-300. Epub 2008 Jun 13.
Loss of MSH2 protein expression is a risk factor in early stage cervical cancer. [J Clin Pathol. 2007]
Loss of MSH2 protein expression is a risk factor in early stage cervical cancer.
Nijhuis ER, Nijman HW, Oien KA, Bell A, ten Hoor KA, Reesink-Peters N, Boezen HM, Hollema H, van der Zee AG. J Clin Pathol. 2007 Jul; 60(7):824-30.
In silico and in vivo splicing analysis of MLH1 and MSH2 missense mutations shows exon- and tissue-specific effects. [BMC Genomics. 2006]
In silico and in vivo splicing analysis of MLH1 and MSH2 missense mutations shows exon- and tissue-specific effects.
Lastella P, Surdo NC, Resta N, Guanti G, Stella A. BMC Genomics. 2006 Sep 22; 7:243. Epub 2006 Sep 22.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.
Cited in Books
NCBI Bookshelf books that cite the current articles.

Recent activity

Clear Turn Off Turn On

Expression of the human mismatch repair gene hMSH2 in normal and neoplastic tiss...
Expression of the human mismatch repair gene hMSH2 in normal and neoplastic tissues.
Cancer Res. 1996 Jan 15 ;56(2):235-40.

PubMed
Black spots concentrate oncogenic asbestos fibers in the parietal pleura. Thorac...
Black spots concentrate oncogenic asbestos fibers in the parietal pleura. Thoracoscopic and mineralogic study.
Am J Respir Crit Care Med. 1996 Jan ;153(1):444-9.

PubMed
Health effects of outdoor air pollution. Committee of the Environmental and Occu...
Health effects of outdoor air pollution. Committee of the Environmental and Occupational Health Assembly of the American Thoracic Society.
Am J Respir Crit Care Med. 1996 Jan ;153(1):3-50.

PubMed
Comparison of carbon dioxide, octenol and a host-odour as mosquito attractants i...
Comparison of carbon dioxide, octenol and a host-odour as mosquito attractants in the Upper Rhine Valley, Germany.
Med Vet Entomol. 1995 Oct ;9(4):377-80.

PubMed
Rhesus incompatibility as a risk factor for schizophrenia in male adults.
Rhesus incompatibility as a risk factor for schizophrenia in male adults.
Arch Gen Psychiatry. 1996 Jan ;53(1):19-24.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Expression of the human mismatch repair gene hMSH2 in normal and neoplastic tissues.

Source

Abstract

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

Medical

Supplemental Content

Save items

Related citations in PubMed

Cited by 17 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information