Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Proc Natl Acad Sci U S A. 1993 May 15;90(10):4557-61.

Methylation induced premeiotically in Ascobolus: coextension with DNA repeat lengths and effect on transcript elongation.

Author information

  • 1Institut de Génétique et Microbiologie, Centre National de la Recherche Scientifique Unité de Recherche Associée 1354, Université Paris-Sud, Orsay, France.

Abstract

The effect of duplications of gene fragments on the triggering of DNA methylation induced premeiotically (MIP) was studied in the sexual progeny of strains harboring, in addition to the resident met2 gene, a fragment of this gene inserted at an ectopic position. Cytosine methylation of the resident gene was checked for each of the eight duplications tested. Methylation was always found and it was coextensive with the length of the duplications. Silencing of the resident gene was triggered by duplications of segments corresponding to the region 5' to the open reading frame, to only the open reading frame, or to segments beginning 0.87-1.2 kb downstream from the transcription start. Silencing was accompanied by either the absence of transcripts or the presence of truncated transcripts, which suggests that methylation acts on transcript elongation.

PMID:
8506299
[PubMed - indexed for MEDLINE]
PMCID:
PMC46551
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk