Abstract
The effects of expressing a chimeric gene consisting of a soybean heat shock gene promoter and a sequence that encodes an enzyme catalyzing the synthesis of a potent phytohormone, the cytokinin iPMP, have been analyzed in transgenic tobacco plants. The production of cytokinin endogenously produced several effects previously undocumented. The differentiation of shoots independent of exogenous cytokinin from heat-treated transgenic plant leaf explants demonstrates that long-term heat treatments do not interfere with complex developmental processes. This extends the potential usefulness of heat shock gene promoters to conditionally express genes during windows of development that span several weeks.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adenosine / analogs & derivatives
-
Adenosine / analysis
-
Alkyl and Aryl Transferases*
-
Cytokinins / biosynthesis*
-
Cytokinins / pharmacology
-
Enzyme Induction
-
Gene Expression Regulation*
-
Genes, Plant / genetics
-
Glycine max / genetics*
-
Heat-Shock Proteins / genetics
-
Hot Temperature
-
Isopentenyladenosine / analogs & derivatives
-
Isopentenyladenosine / analysis
-
Morphogenesis / drug effects
-
Nicotiana / drug effects
-
Nicotiana / genetics
-
Nicotiana / metabolism*
-
Plants, Genetically Modified
-
Plants, Toxic*
-
Promoter Regions, Genetic / genetics
-
Transferases / genetics
-
Transferases / metabolism
Substances
-
Cytokinins
-
Heat-Shock Proteins
-
Isopentenyladenosine
-
zeatin riboside
-
Transferases
-
Alkyl and Aryl Transferases
-
adenylate isopentenyltransferase
-
Adenosine