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Cloning and characterization of a Lambert-Eaton myasthenic syndrome antigen.
Department of Neurology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Lambert-Eaton myasthenic syndrome is a paraneoplastic neuromuscular disorder in which an immune response directed against a small-cell lung tumor crossreacts with antigens in the neuromuscular junction. To isolate and characterize the antigens, we screened a human fetal brain expression library with a high-titer serum from a patient with Lambert-Eaton myasthenic syndrome. This screening resulted in the isolation of a complementary DNA clone encoding an antigen we call myasthenic syndrome antigen B (MysB). Approximately 43% (3 of 7) of Lambert-Eaton myasthenic syndrome sera specifically recognized MysB fusion protein, whereas none of 34 control sera did. The predicted amino acid sequence of this clone shows a high degree of homology to the beta subunit of calcium channel complexes. The MysB pre-messenger RNA is alternatively spliced to yield 3 forms of the protein differing in the domain between two highly conserved alpha-helical segments.
PMID: 8494331 [PubMed - indexed for MEDLINE]
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Cited by 6 PubMed Central articles
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Distinctive modulatory effects of five human auxiliary beta2 subunit splice variants on L-type calcium channel gating.
Takahashi SX, Mittman S, Colecraft HM.
Biophys J. 2003 May; 84(5):3007-21.
[Biophys J. 2003]
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Novel functional properties of Ca(2+) channel beta subunits revealed by their expression in adult rat heart cells.
Colecraft HM, Alseikhan B, Takahashi SX, Chaudhuri D, Mittman S, Yegnasubramanian V, Alvania RS, Johns DC, Marbán E, Yue DT.
J Physiol. 2002 Jun 1; 541(Pt 2):435-52.
[J Physiol. 2002]
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ReviewNeurological paraneoplastic syndromes.
Nath U, Grant R.
J Clin Pathol. 1997 Dec; 50(12):975-80.
[J Clin Pathol. 1997]
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