EMBO J. 1993 May;12(5):2119-28.

Tat-dependent occlusion of the HIV poly(A) site.

Weichs an der Glon C, Ashe M, Eggermont J, Proudfoot NJ.

Source

Sir William Dunn School of Pathology, Oxford, UK.

Abstract

Retroviruses must ensure that poly(A) signals in the 3' LTR are highly active, while identical signals in the 5' LTR are inactive (occluded). In the case of HIV-1, both promoter proximity in the 5' LTR and U3 sequences in the 3' LTR may contribute to this regulation. We have discovered a novel regulatory mechanism for poly(A) site occlusion in HIV-1. When transcription initiation from the HIV promoter is activated by Tat, the HIV poly(A) site is specifically occluded, while other poly(A) sites are unaffected by Tat. Nucleotide signals associated with this Tat effect are immediately adjacent to the AAUAAA sequence of the HIV-1 poly(A) signal. These data suggest that elongating RNA polymerase II, activated by Tat specifically occludes the HIV poly(A) site.

PMID:: 8491200; [PubMed - indexed for MEDLINE]
PMCID:: PMC413433

Free PMC Article

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Distinct transcriptional pathways of TAR-dependent and TAR-independent human immunodeficiency virus type-1 transactivation by Tat. [Virology. 1997]
Distinct transcriptional pathways of TAR-dependent and TAR-independent human immunodeficiency virus type-1 transactivation by Tat.
Yang L, Morris GF, Lockyer JM, Lu M, Wang Z, Morris CB. Virology. 1997 Aug 18; 235(1):48-64.
Tat functions to stimulate the elongation properties of transcription complexes paused by the duplicated TAR RNA element of human immunodeficiency virus 2. [J Mol Biol. 1995]
Tat functions to stimulate the elongation properties of transcription complexes paused by the duplicated TAR RNA element of human immunodeficiency virus 2.
García-Martínez LF, Mavankal G, Peters P, Wu-Baer F, Gaynor RB. J Mol Biol. 1995 Dec 1; 254(3):350-63.
The VP16 transcription activation domain is functional when targeted to a promoter-proximal RNA sequence. [Genes Dev. 1992]
The VP16 transcription activation domain is functional when targeted to a promoter-proximal RNA sequence.
Tiley LS, Madore SJ, Malim MH, Cullen BR. Genes Dev. 1992 Nov; 6(11):2077-87.
Review HIV trans-activation and transcription control mechanisms. [New Biol. 1989]
Review HIV trans-activation and transcription control mechanisms.
Jones KA. New Biol. 1989 Nov; 1(2):127-35.
Review Tackling Tat. [J Mol Biol. 1999]
Review Tackling Tat.
Karn J. J Mol Biol. 1999 Oct 22; 293(2):235-54.

See reviews... See all...

Cited by 9 PubMed Central articles

Review Prospects for antisense peptide nucleic acid (PNA) therapies for HIV. [Expert Opin Biol Ther. 2009]
Review Prospects for antisense peptide nucleic acid (PNA) therapies for HIV.
Pandey VN, Upadhyay A, Chaubey B. Expert Opin Biol Ther. 2009 Aug; 9(8):975-89.
Stem-loop 1 of the U1 snRNP plays a critical role in the suppression of HIV-1 polyadenylation. [RNA. 2000]
Stem-loop 1 of the U1 snRNP plays a critical role in the suppression of HIV-1 polyadenylation.
Ashe MP, Furger A, Proudfoot NJ. RNA. 2000 Feb; 6(2):170-7.
Review Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis. [Microbiol Mol Biol Rev. 1999]
Review Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.
Zhao J, Hyman L, Moore C. Microbiol Mol Biol Rev. 1999 Jun; 63(2):405-45.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (GeneRIF)
Gene records that have the current articles as Reference into Function citations (GeneRIFs). NLM staff reviewing the literature while indexing MEDLINE add GeneRIFs manually.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Tat-dependent occlusion of the HIV poly(A) site.
Tat-dependent occlusion of the HIV poly(A) site.
EMBO J. 1993 May ;12(5):2119-28.

PubMed
Destruction of the CDC28/CLB mitotic kinase is not required for the metaphase to...
Destruction of the CDC28/CLB mitotic kinase is not required for the metaphase to anaphase transition in budding yeast.
EMBO J. 1993 May ;12(5):1969-78.

PubMed
The impact of community mental health centers on the utilization of services.
The impact of community mental health centers on the utilization of services.
Arch Gen Psychiatry. 1977 Apr ;34(4):385-94.

PubMed
Health, safe water and sanitation: a cross-sectional health production function ...
Health, safe water and sanitation: a cross-sectional health production function for central Java, Indonesia.
Bull World Health Organ. 1993 ;71(2):237-45.

PubMed
Acute and late reactions to radiation therapy in patients with collagen vascular...
Acute and late reactions to radiation therapy in patients with collagen vascular diseases.
Cancer. 1993 Jun 1 ;71(11):3744-52.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Tat-dependent occlusion of the HIV poly(A) site.

Source

Abstract

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Cited by 9 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information