Abstract
To augment the chemical potential of the immunological repertoire, a metal ion-binding light chain has been introduced into the murine genome. Mice containing the transgene were subsequently immunized with a fluorescein conjugate. The transgenic light chain was found at a high frequency in the anti-fluorescein memory B-cell compartment. This general method should be applicable to other cofactors and small molecules and should lead to generation of antibodies with unique catalytic activities.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibody Formation
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B-Lymphocytes / immunology*
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Base Sequence
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Crosses, Genetic
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Exons
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Hybridomas / immunology
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Immunoglobulin kappa-Chains / genetics*
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Immunoglobulin kappa-Chains / metabolism*
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Liver / immunology
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Mice
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Mice, Inbred Strains
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Mice, Transgenic
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Oligodeoxyribonucleotides
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Polymerase Chain Reaction / methods
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Restriction Mapping
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Spleen / immunology
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Tumor Cells, Cultured
Substances
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Immunoglobulin kappa-Chains
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Oligodeoxyribonucleotides