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Science. 1993 Apr 9;260(5105):232-4.

Force of single kinesin molecules measured with optical tweezers.

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  • 1Department of Cell Biology, Duke University Medical Center, Durham, NC 27710.

Abstract

Isometric forces generated by single molecules of the mechanochemical enzyme kinesin were measured with a laser-induced, single-beam optical gradient trap, also known as optical tweezers. For the microspheres used in this study, the optical tweezers was spring-like for a radius of 100 nanometers and had a maximum force region at a radius of approximately 150 nanometers. With the use of biotinylated microtubules and special streptavidin-coated latex microspheres as handles, microtubule translocation by single squid kinesin molecules was reversibly stalled. The stalled microtubules escaped optical trapping forces of 1.9 +/- 0.4 piconewtons. The ability to measure force parameters of single macromolecules now allows direct testing of molecular models for contractility.

PMID:
8469975
[PubMed - indexed for MEDLINE]
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