Hidden domains and active site residues in beta-glycanase-encoding gene sequences?

Gene. 1993 Mar 30;125(2):199-204. doi: 10.1016/0378-1119(93)90329-2.

Abstract

Reading-frame corrective shifts in the nucleotide sequence upstream, within, or downstream from the putative coding region of several beta-glycanase-encoding genes reported in the literature reveal hidden active-site residues or even additional domains, including a cellulose-binding domain on a beta-mannanase-encoding gene. These findings also help in assigning, to cellulase family A, two enzymes previously found to lack sequence similarity with known cellulase families.

MeSH terms

  • Amino Acid Sequence
  • Bacteria / enzymology
  • Bacteria / genetics
  • Base Sequence
  • Binding Sites
  • Computer Simulation
  • DNA, Bacterial
  • Glycoside Hydrolases / genetics*
  • Glycoside Hydrolases / metabolism
  • Molecular Sequence Data
  • Open Reading Frames
  • Sequence Homology, Amino Acid

Substances

  • DNA, Bacterial
  • Glycoside Hydrolases
  • glycanase

Associated data

  • GENBANK/M20921
  • GENBANK/M36063
  • SWISSPROT/P04954
  • SWISSPROT/P05522
  • SWISSPROT/P06564
  • SWISSPROT/P07982
  • SWISSPROT/P07983
  • SWISSPROT/P07984
  • SWISSPROT/P07987
  • SWISSPROT/P10474
  • SWISSPROT/P10477
  • SWISSPROT/P15704
  • SWISSPROT/P17901
  • SWISSPROT/P19424
  • SWISSPROT/P19570
  • SWISSPROT/P20847
  • SWISSPROT/P22503
  • SWISSPROT/P22699
  • SWISSPROT/P23659