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J Clin Pathol. 1993 Feb;46(2):145-8.

New phenotypic typing scheme for group B streptococci.

Author information

  • 1Department of Medical Microbiology, St Bartholomew's Hospital Medical College, West Smithfield, London.

Abstract

AIMS:

To develop a new typing system for group B streptococci based on 35S-methionine-labelled protein profiles of bacterial proteins.

METHODS:

377 clinical isolates of group B streptococci were examined by incorporation of 35S-methionine into bacterial proteins under strict anaerobic conditions. After sodium dodecylsulphate-polyacrylamide gel electrophoresis, autoradiography was performed. The patterns produced were visually analysed and categorised into clusters of organisms based on the pattern of band production between 32-46 kilodaltons.

RESULTS:

294 of the typed strains classified into seven different groups designated a-g. 32 strains failed to incorporate 35S-methionine sufficiently to be grouped and 11 strains did not fall into one of the seven identified groups. Typability, reproducibility, and discrimination of the system was evident.

CONCLUSIONS:

This typing system may help to distinguish between colonising and invasive strains of the organism.

PMID:
8459035
PMCID:
PMC501146
[PubMed - indexed for MEDLINE]
Free PMC Article
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