A model for prosthetogenic enzyme amplification assays

S Harbron; M Fisher; B R Rabin

doi:10.1016/0167-4838(93)90230-o

A model for prosthetogenic enzyme amplification assays

Biochim Biophys Acta. 1993 Feb 13;1161(2-3):311-6. doi: 10.1016/0167-4838(93)90230-o.

Authors

S Harbron¹, M Fisher, B R Rabin

Affiliation

¹ London Biotechnology Limited, Department of Biochemistry and Molecular Biology, University College London, UK.

PMID: 8431481
DOI: 10.1016/0167-4838(93)90230-o

Abstract

A mathematical model describing the behaviour of a new class of prosthetogenic enzyme amplification assays is described. The predictions of the model are favourably compared with an enzyme amplification assay for alkaline phosphatase. The model is used to kinetically characterise and optimise the enzyme amplification assay.

MeSH terms

Alkaline Phosphatase / analysis
Alkaline Phosphatase / chemistry
Enzymes / analysis*
Enzymes / chemistry
Flavin-Adenine Dinucleotide
Hydrogen-Ion Concentration
Kinetics
Models, Chemical

Substances

Enzymes
Flavin-Adenine Dinucleotide
Alkaline Phosphatase