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    Biotechniques. 1993 Jan;14(1):42-8.

    Three-color immunofluorescence imaging of Drosophila embryos by laser scanning confocal microscopy.

    Paddock SW, Langeland JA, DeVries PJ, Carroll SB.

    Howard Hughes Medical Institute, Laboratory of Molecular Biology, Madison, WI 53706.

    We present a simple means for triple-labeling biological specimens by immunofluorescence using a laser scanning confocal microscope for imaging with a krypton/argon laser as a light source. Three separate images of fluorescein-, lissamine rhodamine- and cyanine-5-labeled antibodies are collected and subsequently merged to form the triple-labeled image, which is displayed at full-image resolution (24 bit) on a second image processing system. The technique is illustrated using immunofluorescence localization of three segmentation proteins in Drosophila embryos.

    PMID: 8424875 [PubMed - indexed for MEDLINE]

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