Cell kinetics study of upper aerodigestive tract squamous cell carcinoma using bromodeoxyuridine

Ann Otol Rhinol Laryngol. 1993 Jan;102(1 Pt 1):42-6. doi: 10.1177/000348949310200108.

Abstract

Thirty patients treated for squamous cell carcinoma of the upper aerodigestive tract were reviewed for a cell kinetics study using bromodeoxyuridine (BrdU). Fresh specimens were obtained from the primary site of the tumors and incubated in Dulbecco's modified Eagle's medium containing BrdU to label S-phase tumor cells. The tumor specimens were stained immunohistochemically with monoclonal antibody against BrdU. The results demonstrated that the BrdU labeling index (BrdU LI) in these cases (470.3 +/- 118.2) was greater than the BrdU LI in normal mucosa (162 +/- 52.4). In a comparison of the clinical data and histopathologic features, the BrdU LI showed an inverse correlation with patient age (r = -.558; p < .01) and a direct correlation with the frequency of mitoses (r = .411; p < .05). Although the significance of the BrdU LI as a prognostic factor cannot be determined at present, this technique provides a simple in vitro test that avoids the potential hazards of in vivo labeling or the use of radioactive materials.

MeSH terms

  • Adult
  • Aged
  • Bromodeoxyuridine*
  • Carcinoma, Squamous Cell / pathology*
  • Cell Division
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Mouth Neoplasms / pathology*
  • Otorhinolaryngologic Neoplasms / pathology*

Substances

  • Bromodeoxyuridine