New simplified procedures for the extraction and simultaneous high-performance liquid chromatographic analysis of retinol, tocopherols and carotenoids in human serum

J Chromatogr. 1993 Aug 11;617(2):257-64. doi: 10.1016/0378-4347(93)80496-q.

Abstract

A short, simple extraction procedure and a sensitive reversed-phase high-performance liquid chromatographic assay, which utilizes isocratic elution and detection either by a photodiode-array detector or by two detectors set at 300 and 450 nm, have been developed to measure retinol, tocopherols and several carotenoids in human serum simultaneously. By relying on characteristic UV-visible spectra, seventeen carotenoids, retinol and alpha- and gamma-tocopherols were identified in concentrated serum extracts by use of the three-dimensional data mode of a photodiode-array detector. The presence of some recently reported carotenoids in human serum has been confirmed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carotenoids / blood*
  • Chromatography, High Pressure Liquid / methods*
  • Humans
  • Reproducibility of Results
  • Spectrum Analysis
  • Vitamin A / blood*
  • Vitamin E / blood*

Substances

  • Vitamin A
  • Vitamin E
  • Carotenoids