Rapid and highly sensitive high-performance liquid chromatographic method for the determination of histamine and 3-methylhistamine in biological samples using fluorescamine as the derivatizing agent

J Chromatogr. 1993 Aug 11;617(2):233-40. doi: 10.1016/0378-4347(93)80493-n.

Abstract

A highly sensitive and rapid high-performance liquid chromatographic assay for the determination of histamine and 3-methylhistamine in biological samples using 1-methylhistamine as the internal standard is described. Samples were purified and concentrated on cation-exchange columns and derivatized with fluorescamine. The lower detection limit was 20 pg on-column. Linearity was demonstrated up to 20 ng on-column. The samples could be derivatized simultaneously before injection and were stable for 7 days. The method was used for the determination of histamine and related compounds in coronary perfusates, extracts of homogenized rat hearts, and supernatants of stimulated peritoneal mast cells.

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid / methods*
  • Fluorescamine / chemistry*
  • Histamine / analysis*
  • Male
  • Mast Cells / chemistry
  • Methylhistamines / analysis*
  • Myocardium / chemistry
  • Rats
  • Rats, Wistar
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Methylhistamines
  • Fluorescamine
  • 3-methylhistamine
  • Histamine