Abstract
To define the interaction site of T cell antigen receptors (TCRs) with the superantigen of Mycoplasma arthritidis (MAM), we characterized the TCRs of human MAM-reactive T cell lines and T cell clones. Most MAM-reactive T cells express the V beta 17 gene segment; however, other V beta may also be used and not all V beta 17+ TCRs are MAM-reactive. Alignment of MAM-reactive and MAM-nonreactive V beta sequences suggests that MAM does not interact with a single site encoded by a V beta segment but that several sites may be involved or that other TCR elements contribute to formation of the MAM recognition site.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens
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Antigens, Bacterial
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Binding Sites / genetics
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Cell Line
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Conserved Sequence
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Humans
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Mice
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Mitogens / immunology*
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Molecular Sequence Data
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Mycoplasma / immunology*
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Proteins
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Receptors, Antigen, T-Cell, alpha-beta / genetics
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Receptors, Antigen, T-Cell, alpha-beta / immunology
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Sequence Alignment
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Superantigens*
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T-Lymphocytes / immunology*
Substances
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Antigens
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Antigens, Bacterial
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Mitogens
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Mycoplasma arthritidis mitogen
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Proteins
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Receptors, Antigen, T-Cell, alpha-beta
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Superantigens