Simian immunodeficiency virus-specific cytotoxic T lymphocytes in rhesus monkeys: characterization and vaccine induction

Semin Immunol. 1993 Jun;5(3):215-23. doi: 10.1006/smim.1993.1025.

Abstract

An effective HIV vaccine should be capable of eliciting virus-specific cytotoxic T lymphocytes (CTL). We have characterized the cellular and molecular features of a simian immunodeficiency virus of macaques (SIVmac) gag-specific CTL response in rhesus monkeys. We have shown that SIVmac-infected rhesus monkeys expressing the major histocompatibility complex (MHC) class I molecule Mamu-A*01 develop a SIVmac gag-specific CTL response which recognizes a 9 amino acid fragment of the gag protein in association with Mamu-A*01. Moreover, this peptide/MHC class I recognition is mediated by T cell receptors (TCR) employing a predominant V beta gene family and J beta gene. Using this understanding of a SIVmac-specific CTL response, we have shown that SIVmac-specific CTL can be elicited through three novel approaches to vaccination: a recombinant viral vector, a recombinant bacterial vector and a peptide vaccine. These studies illustrate the utility of the SIV/macaque model in AIDS vaccine research.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Gene Products, env / immunology
  • Histocompatibility Antigens Class I / immunology
  • Liposomes
  • Macaca mulatta / immunology*
  • Mycobacterium bovis / genetics
  • Mycobacterium bovis / immunology
  • Parainfluenza Virus 1, Human / immunology
  • Peptide Fragments / immunology
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / immunology
  • Retroviridae Proteins, Oncogenic / immunology
  • Simian Acquired Immunodeficiency Syndrome / immunology*
  • Simian Acquired Immunodeficiency Syndrome / pathology
  • Simian Immunodeficiency Virus / immunology*
  • T-Lymphocytes, Cytotoxic / immunology*
  • T-Lymphocytes, Cytotoxic / pathology
  • Vaccination
  • Vaccines, Synthetic / immunology
  • Vaccinia virus / genetics
  • Viral Fusion Proteins*
  • Viral Vaccines*

Substances

  • Gene Products, env
  • Histocompatibility Antigens Class I
  • Liposomes
  • Peptide Fragments
  • Receptors, Antigen, T-Cell
  • Retroviridae Proteins, Oncogenic
  • Vaccines, Synthetic
  • Viral Fusion Proteins
  • Viral Vaccines
  • transmembrane protein, Simian immunodeficiency virus