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Gene. 1993 Aug 16;130(1):151-2.

M13 and pUC vectors with new unique restriction sites for cloning.

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  • 1Institute of Molecular Genetics, Czech Academy of Sciences, Prague.


Several vectors based on the widely used phage M13 and plasmid pUC were constructed. The vectors contain polylinkers (MCS) for DNA insertions with several new restriction sites (e.g., ApaI, NotI, StuI, SacII). Moreover, the NarI site in the nonessential part of the vector molecule was changed into a BssHII site, so that the NarI site in the MCS became unique.

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