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Molecular cloning of a human beta 3-adrenergic receptor cDNA.
Lelias JM,
Kaghad M,
Rodriguez M,
Chalon P,
Bonnin J,
Dupre I,
Delpech B,
Bensaid M,
LeFur G,
Ferrara P, et al.
Laboratoire de Biologie Moleculaire, Sanofi Elf Biorecherches, Labege, France.
We report the molecular cloning of a beta 3-adrenergic receptor [beta 3-AR] cDNA from human brown adipose tissue. The cDNA-encoded protein is identical to the previously cloned beta 3-AR but with 6 additional amino acids at the C-terminus. The C-terminus is shared by the beta 3 receptors expressed in human neuroblastoma cells [SK-N-MC] [Mol. Pharmacol. 42 (1992) 964-970]. Furthermore, using a polymerase chain reaction strategy we have cloned and sequenced the beta 3-AR introns. Sequence analysis demonstrates that the human beta 3-AR gene comprises at least 3 exons and 2 introns and that the most abundant beta 3-AR transcripts encode a protein with an exon 3-derived C-terminus. Interestingly, although a similar organization has been found in rodent genes, the rat beta 3-AR transcripts encode a receptor with an exon 2-derived C-terminus.
PMID: 8389717 [PubMed - indexed for MEDLINE]
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