Format

Send to:

Choose Destination
See comment in PubMed Commons below
Obstet Gynecol. 1993 Apr;81(4):536-40.

The superoxide generation of neutrophils in normal and preeclamptic pregnancies.

Author information

  • 1Department of Gynecology and Obstetrics, Faculty of Medicine, Kyushu University, Japan.

Abstract

OBJECTIVE:

To determine whether preeclampsia enhances superoxide generation of neutrophils.

METHODS:

Samples were obtained from 12 normal nonpregnant, 15 normal pregnant, and 11 preeclamptic women. The superoxide production of neutrophils was measured by the superoxide dismutase-inhibitable reduction of ferricytochrome C at 550-540 nm using a dual-wavelength spectrophotometer.

RESULTS:

N-formyl-methionyl-leucyl-phenylalanine (FMLP)-induced superoxide production of neutrophils was significantly higher in preeclamptics than in normal nonpregnant and pregnant subjects, whereas phorbol 12-myristate 13-acetate-induced superoxide production did not differ among them. When neutrophils prepared from normal nonpregnant women were preincubated with sera from the study groups, sera from preeclamptics enhanced the FMLP-induced superoxide production to about twice that of the other subjects. The serum-enhancing factor appeared to be a heat-labile protein, different from other known activators.

CONCLUSION:

Preeclampsia is characterized by the presence of a neutrophil activator that enhances superoxide production. This enhanced production may contribute to the pathophysiologic changes observed in preeclampsia.

PMID:
8384706
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk