The murine myeloperoxidase promoter contains several functional elements, one of which binds a cell type-restricted transcription factor, myeloid nuclear factor 1 (MyNF1)

Mol Cell Biol. 1993 Apr;13(4):2141-51. doi: 10.1128/mcb.13.4.2141-2151.1993.

Abstract

The myeloperoxidase (MPO) gene is expressed specifically in myeloid cells. There is significant homology between the murine and human MPO genes in the 1.6-kb region located upstream of the murine MPO transcription initiation sites. 5',3', and internal deletions of this DNA segment localized several cis-acting DNA elements in the murine MPO promoter which are functional in 32D cl3 cells, a murine myeloblast cell line which expresses MPO. These DNA elements did not function well in mouse L-cell fibroblasts. Additional mutagenesis of the most active promoter region allowed the delimitation of a functional 20-bp segment. Mutation of the enhancer core motif within this segment was functionally deleterious, and an oligonucleotide containing these base pairs increased the activity of a minimal promoter. This same oligonucleotide, but not a mutant variant, could bind a set of nuclear proteins, myeloid nuclear factors 1 alpha and 1 beta (MyNF1 alpha and -1 beta), present in 32D cl3 cells but absent from L cells, murine erythroleukemia cells, and SP2 lymphoid cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • DNA Mutational Analysis
  • DNA-Binding Proteins / metabolism*
  • Gene Expression Regulation, Enzymologic*
  • Hematopoietic Stem Cells / physiology*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism*
  • Oligodeoxyribonucleotides / chemistry
  • Peroxidase / genetics*
  • Promoter Regions, Genetic*
  • RNA, Messenger / genetics
  • Sequence Deletion
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism*

Substances

  • DNA-Binding Proteins
  • Mynf1 protein, mouse
  • Nuclear Proteins
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Transcription Factors
  • Peroxidase

Associated data

  • GENBANK/X15378