Abstract
The full activity of a recombination initiation site located 5' of HIS4 requires the binding of the transcription factors RAP1, BAS1, and BAS2. Two RAP1 binding sites can substitute for the wild-type initiation site. A 51-bp region of telomeric DNA inserted upstream of either HIS4 or ARG4 very strongly stimulates recombination. We suggest that the ability of transcription factors to induce recombination is a consequence of an altered chromatin structure that favors the entry of proteins that initiate recombination, rather than an effect of these factors on transcription.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Chromatin / ultrastructure
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Crosses, Genetic
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DNA Mutational Analysis
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Fungal Proteins / metabolism
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GTP-Binding Proteins / metabolism
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Gene Expression Regulation, Fungal
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Genes, Fungal / genetics*
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Homeodomain Proteins*
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Molecular Sequence Data
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Recombination, Genetic*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Sequence Deletion
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Telomere
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Trans-Activators / metabolism
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Transcription Factors / metabolism*
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Transcription, Genetic
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rap GTP-Binding Proteins
Substances
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BAS1 protein, S cerevisiae
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Chromatin
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Fungal Proteins
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Homeodomain Proteins
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PHO2 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Trans-Activators
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Transcription Factors
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GTP-Binding Proteins
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rap GTP-Binding Proteins