Iontophoretic transport through porous membranes using scanning electrochemical microscopy: application to in vitro studies of ion fluxes through skin

Anal Chem. 1993 Jun 1;65(11):1537-45. doi: 10.1021/ac00059a010.

Abstract

Scanning electrochemical microscopy (SECM) is used to map localized iontophoretic fluxes of electroactive species through porous membranes. A method is described that allows both the rate of transport of species from a microscopic pore and the pore's diameter to be measured. SECM images and analyses of synthetic porous membranes (track-etched polycarbonate and mica membranes) and hairless mouse skin are reported. Preliminary analysis of SECM images of the mouse skin indicates that a significant percentage of the iontophoretic flux occurs through pores associated with hair follicles.

MeSH terms

  • Aluminum Silicates / chemistry
  • Animals
  • Biological Transport
  • Diffusion
  • Ferricyanides / metabolism*
  • Iontophoresis*
  • Male
  • Membranes, Artificial
  • Mice
  • Mice, Hairless
  • Microscopy, Electron, Scanning*
  • Oxidation-Reduction
  • Polycarboxylate Cement / chemistry
  • Skin / metabolism*

Substances

  • Aluminum Silicates
  • Ferricyanides
  • Membranes, Artificial
  • Polycarboxylate Cement
  • hexacyanoferrate III
  • polycarbonate
  • mica