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Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6213-7.

Complex formation between RAS and RAF and other protein kinases.

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  • 1Cold Spring Harbor Laboratory, NY 11724.

Abstract

We used a Saccharomyces cerevisiae genetic system to detect the physical interaction of RAS and RAF oncoproteins. We also observed interaction between RAS and byr2, a protein kinase implicated as a mediator of the Schizosaccharomyces pombe ras1 protein. Interaction with RAS required only the N-terminal domains of RAF or byr2 and was disrupted by mutations in either the guanine nucleotide-binding or effector-loop domains of RAS. We observed interaction between MEK (a kinase that phosphorylates mitogen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed.

PMID:
8327501
[PubMed - indexed for MEDLINE]
PMCID:
PMC46898
Free PMC Article
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