Plasma membrane: rapid isolation and exposure of the cytoplasmic surface by use of positively charged beads

Science. 1977 Jan 21;195(4275):302-4. doi: 10.1126/science.831278.

Abstract

Erythrocytes were ionically attached to polylysine-coated beads 30 micrometers in diameter. The binding of the cells was so tenacious that lysis or disruption of the attached cells left the beads covered by plasma membranes whose cytoplasmic surface was exposed and accessible for further analysis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Fractionation
  • Cell Membrane* / physiology
  • Cell Membrane* / ultrastructure
  • Erythrocyte Membrane* / analysis
  • Erythrocyte Membrane* / physiology
  • Erythrocyte Membrane* / ultrastructure
  • Erythrocytes* / ultrastructure
  • Glass
  • Humans
  • Membrane Lipids / analysis
  • Membrane Proteins / analysis
  • Methods
  • Phospholipids / analysis

Substances

  • Membrane Lipids
  • Membrane Proteins
  • Phospholipids