Gene. 1993 Dec 22;136(1-2):365-8.

A novel transcript from a pseudogene for human glucocerebrosidase in non-Gaucher disease cells.

Imai K, Nakamura M, Yamada M, Asano A, Yokoyama S, Tsuji S, Ginns EI.

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Department of Biochemistry, Shimane Medical University, Izumo, Japan.

Abstract

Human glucocerebrosidase (GC)-encoding cDNA clones were isolated from a promyelocytic HL-60 cDNA library and analyzed. A novel cDNA clone was found to originate from a gene referred to as a GC pseudogene. Using the polymerase chain reaction (PCR) with primers specific for the GC pseudogene, we found that all the human cell lines examined, HL-60, K-562, WI-38, HepG2 and HeLa, expressed a pseudogene transcript. In vitro translation of RNA synthesized by transcription of the pseudogene cDNA produced a polypeptide of approximately 30 kDa.

PMID:: 8294033; [PubMed - indexed for MEDLINE]

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A novel transcript from a pseudogene for human glucocerebrosidase in non-Gaucher...
A novel transcript from a pseudogene for human glucocerebrosidase in non-Gaucher disease cells.
Gene. 1993 Dec 22 ;136(1-2):365-8.

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