Abstract
1. Mouse plasma creatinine measured by fuller's earth (15.1 +/- 0.5(41) microM, mean +/- SE) exceeded the same pools read by HPLC [7.5 +/- 0.2(41); P < 0.001]. 2. For mouse urine, the fuller's earth method (3.26 +/- 0.21 mM) slightly exceeded the HPLC method (2.65 +/- 0.18; P < 0.001) with good correlation (r = 0.915, P < 0.001). 3. An enzymatic method (creatinine PAP, Boehringer-Mannheim Diagnostics) for plasma creatinine (5.2 +/- 0.3 microM) was equivalent to HPLC (6.1 +/- 0.1, NS) but with poor correlation (r = 0.23, NS). 4. Creatinine in the urine agreed closely between HPLC (2.84 +/- 0.12 mM) and the enzymatic procedure (2.95 +/- 0.17, NS) with good correlation (r = 0.845, P < 0.001).
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aluminum Compounds*
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Amidohydrolases
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Animals
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Chromatography, High Pressure Liquid
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Creatinine / analysis*
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Creatinine / blood
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Creatinine / urine
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Female
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Magnesium Compounds*
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Male
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Mice
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Mice, Inbred C57BL / blood
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Mice, Inbred C57BL / metabolism*
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Mice, Inbred C57BL / urine
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Mice, Mutant Strains
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Oxidoreductases, N-Demethylating
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Sarcosine Oxidase
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Silicates*
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Ureohydrolases
Substances
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Aluminum Compounds
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Magnesium Compounds
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Silicates
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Fuller's Earth
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Creatinine
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Oxidoreductases, N-Demethylating
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Pipox protein, mouse
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Sarcosine Oxidase
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Amidohydrolases
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creatininase
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Ureohydrolases
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creatinase