Creatinine assay by the fuller's earth procedure or by enzymatic determination is adequate for urine but not plasma of mice

Comp Biochem Physiol B. 1993 Nov;106(3):685-9. doi: 10.1016/0305-0491(93)90150-4.

Abstract

1. Mouse plasma creatinine measured by fuller's earth (15.1 +/- 0.5(41) microM, mean +/- SE) exceeded the same pools read by HPLC [7.5 +/- 0.2(41); P < 0.001]. 2. For mouse urine, the fuller's earth method (3.26 +/- 0.21 mM) slightly exceeded the HPLC method (2.65 +/- 0.18; P < 0.001) with good correlation (r = 0.915, P < 0.001). 3. An enzymatic method (creatinine PAP, Boehringer-Mannheim Diagnostics) for plasma creatinine (5.2 +/- 0.3 microM) was equivalent to HPLC (6.1 +/- 0.1, NS) but with poor correlation (r = 0.23, NS). 4. Creatinine in the urine agreed closely between HPLC (2.84 +/- 0.12 mM) and the enzymatic procedure (2.95 +/- 0.17, NS) with good correlation (r = 0.845, P < 0.001).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aluminum Compounds*
  • Amidohydrolases
  • Animals
  • Chromatography, High Pressure Liquid
  • Creatinine / analysis*
  • Creatinine / blood
  • Creatinine / urine
  • Female
  • Magnesium Compounds*
  • Male
  • Mice
  • Mice, Inbred C57BL / blood
  • Mice, Inbred C57BL / metabolism*
  • Mice, Inbred C57BL / urine
  • Mice, Mutant Strains
  • Oxidoreductases, N-Demethylating
  • Sarcosine Oxidase
  • Silicates*
  • Ureohydrolases

Substances

  • Aluminum Compounds
  • Magnesium Compounds
  • Silicates
  • Fuller's Earth
  • Creatinine
  • Oxidoreductases, N-Demethylating
  • Pipox protein, mouse
  • Sarcosine Oxidase
  • Amidohydrolases
  • creatininase
  • Ureohydrolases
  • creatinase