Send to

Choose Destination
See comment in PubMed Commons below
Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):128-32.

Strain-specific and tissue-specific expression of mouse mast cell secretory granule proteases.

Author information

  • 1Department of Medicine, Harvard Medical School, Boston, MA 02115.


As assessed by RNA blot analyses with gene-specific probes, we report that the perivascular connective tissue mast cells (CTMCs) in the ear and skin of BALB/cJ mice contain abundant levels of the mouse mast cell protease (mMCP) 7 transcript, in addition to those protease transcripts present in their serosal mast cells (SMCs). High levels of the mMCP-7 transcript also were detected in the ears of WBB6F1/J(-)+/+, WCB6F1/J(-)+/+, WB/ReJ(-)+/+, and WC/ReJ(-)+/+ mice. However, the ears of these four strains and the SMCs from the WCB6F1/J(-)+/+ strain but not the BALB/cJ strain also contained high steady-state levels of the mMCP-2 transcript. The mMCP-2, mMCP-4, mMCP-5, mMCP-6, and mMCP-7 transcripts were not detected in the ears of mast-cell-deficient WBB6F1/J-W/Wv and WCB6F1/J-Sl/Sld mice, indicating that mast cells were the source of these protease transcripts in the +/+ animals. When immunohistochemical analyses of serial sections of ear and skin from WBB6F1/J(-)+/+ mice were performed with anti-mMCP-2 IgG and anti-mMCP-5 IgG, the perivascular CTMCs in these tissues were found to express both mMCP-2 and mMCP-5 in their granules. The prominent expression of mMCP-7 in constitutive perivascular CTMCs indicates that this mast cell has an extended protease phenotype relative to the SMCs of the same strains. Further, the perivascular CTMCs and SMCs of the +/+ strains differ from those in BALB/cJ mice in their prominent expression of mMCP-2.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk