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    Hum Genet. 1994 Jan;93(1):63-6.

    Isolation and characterization of the human genomic locus coding for the putative metastasis control gene nm23-H1.

    Dooley S, Seib T, Engel M, Theisinger B, Janz H, Piontek K, Zang KD, Welter C.

    Source

    Institut für Humangenetik, Universität des Saarlandes, Homburg-Saar, Germany.

    Abstract

    Nm23-H1 gene expression is inversely correlated with tumor metastatic potential in certain tumors, including melanomas, breast carcinomas, and hepatocellular carcinomas. Using nm23-H1 c-DNA primer and genomic polymerase chain reaction (PCR) amplification, we purified three PCR fragments (one of 4kb and two of 2 kb) covering the whole human genomic locus of the gene (8.460bp). We recombined the PCR products into pUC18 and produced a restriction map to perform subcloning. Complete sequencing of genomic PCR fragments, including the whole coding region of nm23-H1, revealed that the gene consists of five exons and four introns spanning 8.5kb. A sequence homology analysis between human nm23-H1 and the homolog gene of the rat (NDP-K beta) shows that exon-intron boundaries are well conserved between these two species.

    PMID:
    8270257
    [PubMed - indexed for MEDLINE]

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