[Basic study of CA125 measurement using a newly developed "SD-8729" IRMA kit]

A Yamaguchi; M Kobayashi; T Imai; H Otake; K Matsubara; N Hatori; K Tomiyoshi; T Inoue; K Endo

[Basic study of CA125 measurement using a newly developed "SD-8729" IRMA kit]

Kaku Igaku. 1993 Oct;30(10):1245-52.

[Article in Japanese]

Authors

A Yamaguchi¹, M Kobayashi, T Imai, H Otake, K Matsubara, N Hatori, K Tomiyoshi, T Inoue, K Endo

Affiliation

¹ Department of Nuclear Medicine, School of Medicine, Gunma University.

PMID: 8264116

Abstract

"SD-8729" is a one-step IRMA kit employing OC125 antibody as 125I-labeled tracer and M-11 antibody as an immunoadsorbent. Higher bound-radioactivity to beads was observed with shorter incubation time than that of a currently used CA125 IRMA kit which employed OC125 antibody both as 125I-labeled ligand and immunoadsorbent attached to beads. Almost identical CA125 values were obtained by using two kits. The antigenic nature recognized by the M-11 antibody seems substantially different from those of 130-22 or 145-9 antibodies recognizing CA130 antigen.

Publication types

English Abstract

MeSH terms

Antibodies, Monoclonal
Antigens, Tumor-Associated, Carbohydrate / blood*
Biomarkers, Tumor / blood*
Evaluation Studies as Topic
Female
Humans
Immunoradiometric Assay / methods*
Ovarian Neoplasms / diagnosis
Reagent Kits, Diagnostic / standards*

Substances

Antibodies, Monoclonal
Antigens, Tumor-Associated, Carbohydrate
Biomarkers, Tumor
Reagent Kits, Diagnostic