We perfected the narrow spectral band fundus photographic system using interference filters at the wavelengths of 417, 478, 500, 530, 547, 570, 589, 607, 628 and 648 nm. Tests about the light penetration of filters and exposure of various brand films were made on this system. Studies of the contrast of fundal tissues and structures under the different narrow spectral band light were made on 43 Chinese fellow eyes. The results indicates that the interference filters of 570 nm have the highest light penetration. Kodak and Gongyuan films (make in China) are the optimal ones. Narrow spectral band fundus photography can eliminate the chromatic aberration and therefore improve the documentation of fundal details, enhance the contrast of pictures as compared with routine white light fundus photography.