Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Cryobiology. 1993 Oct;30(5):519-23.

Protection of freeze-dried Escherichia coli by trehalose upon exposure to environmental conditions.

Author information

  • 1U.S. Environmental Protection Agency, Environmental Research Laboratory, Corvallis, Oregon.

Abstract

Freeze-dried cultures of wild-type and genetically engineered strains of Escherichia coli lost their colony-forming ability upon exposure to air, visible light, and certain relative humidity levels. Both strains could be maximally protected from these lethal effects with 100 mM trehalose, a concentration calculated to just saturate the interphospholipid spaces in the cell membrane, thus preserving the liquid-crystalline structure. The trehalose protection was observed for at least 96 h. Trehalose increased viability as much as 2000-4000% over nontreated populations. In all cases, exposure to environmental conditions was more damaging to the genetically engineered strain.

PMID:
8252918
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk