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1: Biotechniques. 1993 Oct;15(4):668-70, 672.Links

Funnel-well SDS-PAGE: a rapid technique for obtaining sufficient quantities of low-abundance proteins for internal sequence analysis.

Lombard-Platet G, Jalinot P.

Laboratoire de Biologie Moléculaire et Cellulaire, Ecole Normale Supérieure de Lyon.

We report a modified sodium dodecyl sulfate polyacrylamide gel electrophoresis method that permits up to a 60-fold concentration factor, without significant loss of protein. This method leads to very efficient concentration of low-abundance proteins from partially purified fractions or very dilute protein solution. Furthermore, it permits in situ enzymatic digestion and consequently increases the probability of obtaining a suitable internal sequence.

PMID: 8251169 [PubMed - indexed for MEDLINE]