This double-blind, randomized, controlled clinical trial was designed to evaluate the efficacy and soft tissue effects of nightguard (mouthguard) vital bleaching. Subjects were assigned to one of two treatment groups, overnight (insertion of mouthguard at bedtime) or 3-hour (replenishment of test solution at hourly intervals for a total of 3 hours exposure daily). The experimental solutions included two products that are specifically designed for tooth bleaching, one product that is marketed as an oral antiseptic, and a control solution (glycerin). Each subject participated for a 3-week period. Tooth color analysis (shade determination) was done at baseline and the end of the treatment. Gingival and Plaque Indices were recorded at baseline and at the end of each week of study. Results indicated that the bleaching treatment was effective in most cases and caused no tissue inflammation or significant tooth sensitivity. The mean Gingival and Plaque Indices were generally lower (denoting improvement) at the end of treatment. Patient satisfaction with the procedure was high.