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J Bacteriol. 1993 Nov;175(21):7115-8.

Use of Salmonella phage P22 for transduction in Escherichia coli.

Author information

  • 1Department of Microbiology, University of Sydney, New South Wales, Australia.

Abstract

A cosmid (pPR1347) carrying both the rfb gene cluster and the rfc gene of a Salmonella group B serovar has been constructed; Escherichia coli K-12 strains carrying this cosmid produce long-chain O antigen, are sensitive to phage P22, and can be transduced by P22. Some of the benefits of P22 transduction are now available for studying E. coli and potentially other genera.

PMID:
8226656
[PubMed - indexed for MEDLINE]
PMCID:
PMC206843
Free PMC Article
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