1. Tryptophan has been shown to inhibit dopa-oxidation by melanosomal tyrosinase. 2. The inhibition is of mixed-type with Ki = 1.6 x 10(-3) M. 3. Tryptophan does not interact with the oxidation product of the dopa-oxidase reaction. 4. Neither oxygen nor hydroxyl radicals are involved in the inhibition found in presence of tryptophan. 5. Tryptophan, like dopa, also inhibits tyrosine hydroxylase and dopa-oxidase activity of melanosomal tyrosinase and its inhibitory mechanism differs from inhibition due to non-substrate type compounds like cysteine, ascorbic acid. 6. These experiments together with previous findings suggest that the status of tryptophan may be similar to that of dopa in relation to regulation of melanogenesis.