Mechanisms involved in the cardiovascular-renal actions of nitric oxide inhibition

Hypertension. 1994 Jun;23(6 Pt 2):951-6. doi: 10.1161/01.hyp.23.6.951.

Abstract

The roles of the sympathetic nervous system, angiotensin II, and arginine vasopressin in the cardiovascular-renal responses to nitric oxide synthesis inhibition were examined in eight conscious dogs equipped with arterial and venous catheters and a nonoccluding bladder catheter. Nitric oxide inhibition was achieved by intravenous infusion of NG-nitro-L-arginine methyl ester (L-NAME) at 37.1 nmol/kg per minute for 140 minutes in the control group. The same dogs, after a 1-week recovery, were pretreated for 2 days with either prazosin for alpha 1 blockade, prazosin plus propranolol for alpha 1 plus beta blockade, L-158,809 for angiotensin receptor blockade, or d(CH2)Tyr(Me)arginine vasopressin for vasopressin-V1 blockade, and the L-NAME infusion was repeated. After 140 minutes of L-NAME infusion into the control group, mean arterial pressure and renal vascular resistance had increased 16% and 71%, and renal blood flow, glomerular filtration rate, urine flow, and urinary sodium excretion had decreased 33%, 16%, 61%, and 64%, respectively. The decrement in renal blood flow and glomerular filtration during L-NAME administration was unaffected by any of the neurohumoral blockers. During V1 blockade L-NAME resulted in only a 3% increase in arterial pressure, attenuation of the renal vascular resistance response, and almost total elimination of the decrease in urine flow. During angiotensin blockade the L-NAME-induced increase in arterial pressure was markedly attenuated, and the decrease in urinary sodium excretion was attenuated in the alpha 1 plus beta blockade group.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arginine / analogs & derivatives
  • Arginine / pharmacology
  • Arginine Vasopressin / antagonists & inhibitors
  • Blood Pressure
  • Cardiovascular Physiological Phenomena*
  • Diuresis
  • Dogs
  • Glomerular Filtration Rate
  • Kidney / physiology*
  • Male
  • NG-Nitroarginine Methyl Ester
  • Natriuresis
  • Nitric Oxide / antagonists & inhibitors
  • Nitric Oxide / physiology*
  • Renal Circulation
  • Vascular Resistance

Substances

  • Arginine Vasopressin
  • Nitric Oxide
  • Arginine
  • NG-Nitroarginine Methyl Ester