Abstract
Using an expression library, we have isolated yeast genes activated in the presence of the yeast CCAAT box-binding protein HAP2. One of these genes, SDH3, encodes the cytochrome b560 subunit of respiratory complex II. The SDH3 protein contains three potential transmembrane domains and is more than 30% identical to bovine cytochrome b560 and to a mitochondrially encoded protein from Marchantia polymorpha. Disruption of SDH3 shows that this gene is required for growth on non-fermentable carbon sources. Expression of SDH1, SDH3, and SDH4 is activated in the presence of the HAP2 transcriptional activator.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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CCAAT-Binding Factor*
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Cattle
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Cytochrome b Group / biosynthesis
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Cytochrome b Group / genetics*
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DNA Transposable Elements
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Electron Transport Complex II
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Fungal Proteins / metabolism
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Gene Expression Regulation, Fungal*
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Gene Library
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Genes, Fungal*
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Molecular Sequence Data
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Multienzyme Complexes / biosynthesis
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Multienzyme Complexes / genetics*
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Oxidoreductases / biosynthesis
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Oxidoreductases / genetics*
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Plasmids
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Protein Structure, Secondary
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Recombinant Fusion Proteins / biosynthesis
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics*
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Sequence Homology, Amino Acid
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Succinate Dehydrogenase / biosynthesis
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Succinate Dehydrogenase / genetics*
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Transcription Factors / metabolism
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beta-Galactosidase / biosynthesis
Substances
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CCAAT-Binding Factor
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Cytochrome b Group
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DNA Transposable Elements
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Fungal Proteins
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Multienzyme Complexes
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Recombinant Fusion Proteins
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Transcription Factors
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cytochrome b560
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Oxidoreductases
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Electron Transport Complex II
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Succinate Dehydrogenase
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beta-Galactosidase