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1: Science. 1994 May 27;264(5163):1322-6.Click here to read Links

Interaction of MHC class I molecules with the transporter associated with antigen processing.

Suh WK, Cohen-Doyle MF, Fruh K, Wang K, Peterson PA, Williams DB.

Department of Biochemistry, University of Toronto, Ontario, Canada.

The transporter associated with antigen processing (TAP) delivers cytosolic peptides into the endoplasmic reticulum (ER) where they bind to nascent class 1 histocompatibility molecules. Class 1-peptide complexes are then displayed at the cell surface for recognition by cytotoxic T lymphocytes. Immunoprecipitation of either TAP or class 1 molecules revealed an association between the transporter and diverse class 1 products. TAP bound preferentially to heterodimers of the class 1 heavy chain and beta 2-microglobulin, and the complex subsequently dissociated in parallel with transport of class 1 molecules from the ER to the Golgi apparatus. The TAP-class 1 complexes could also be dissociated in vitro by the addition of class 1-binding peptides. The association of class 1 molecules with TAP likely promotes efficient capture of peptides before their exposure to the lumen of the ER.

PMID: 8191286 [PubMed - indexed for MEDLINE]