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Braz J Med Biol Res. 1994 Jan;27(1):81-9.

Properties of phosphorylated glial fibrillary acidic protein in brain slices of guinea pig, mouse and rat.

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  • 1Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.


1. Brain micro-slices from guinea pig, mouse and rat were incubated in Krebs-Ringer Na-HEPES buffered medium containing [32P]-phosphate and characterized by immunoblotting with polyclonal antibody to glial fibrillary acidic protein (GFAP). 2. GFAP presented small differences in two-dimensional electrophoretic mobility. 3. The phosphorylation of GFAP was dependent on Ca2+ in the incubation medium in adult animals. 4. Both the immunocontent and level of phosphorylation of GFAP were higher in hippocampus than in cerebral cortex of all three species.

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