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Gene structure of the Helicobacter pylori cytotoxin and evidence of its key role in gastric disease.
Telford JL,
Ghiara P,
Dell'Orco M,
Comanducci M,
Burroni D,
Bugnoli M,
Tecce MF,
Censini S,
Covacci A,
Xiang Z, et al.
Department of Molecular Biology, Immunobiological Research Institute Siena, Italy.
The gram negative, microaerophilic bacterium Helicobacter pylori colonizes the human gastric mucosa and establishes a chronic infection that is tightly associated with atrophic gastritis, peptic ulcer, and gastric carcinoma. Cloning of the H. pylori cytotoxin gene shows that the protein is synthesized as a 140-kD precursor that is processed to a 94-kD fully active toxin. Oral administration to mice of the purified 94-kD protein caused ulceration and gastric lesions that bear some similarities to the pathology observed in humans. The cloning of the cytotoxin gene and the development of a mouse model of human gastric disease will provide the basis for the understanding of H. pylori pathogenesis and the development of therapeutics and vaccines.
PMID: 8163943 [PubMed - indexed for MEDLINE]
PMCID: PMC2191472
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Cited by over 100 PubMed Central articles
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Serum antibodies positivity to 12 Helicobacter pylori virulence antigens in patients with benign or malignant gastroduodenal diseases--cross-sectional study.
Filipec Kanizaj T, Katicić M, Presecki V, Gasparov S, Colić Cvrlje V, Kolarić B, Mrzljak A.
Croat Med J. 2009 Apr; 50(2):124-32.
[Croat Med J. 2009]
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Concurrent genotyping of Helicobacter pylori virulence genes and human cytokine SNP sites using whole genome amplified DNA derived from minute amounts of gastric biopsy specimen DNA.
Ryberg A, Borch K, Sun YQ, Monstein HJ.
BMC Microbiol. 2008 Oct 8; 8:175. Epub 2008 Oct 8.
[BMC Microbiol. 2008]
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Sphingomyelin functions as a novel receptor for Helicobacter pylori VacA.
Gupta VR, Patel HK, Kostolansky SS, Ballivian RA, Eichberg J, Blanke SR.
PLoS Pathog. 2008 May 23; 4(5):e1000073. Epub 2008 May 23.
[PLoS Pathog. 2008]
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