Structural elucidation of the capsular polysaccharide expressed by Escherichia coli O20:K83:H26 by high resolution NMR spectroscopy

D V Whittaker; L A Parolis; H Parolis

doi:10.1016/0008-6215(94)80069-3

Structural elucidation of the capsular polysaccharide expressed by Escherichia coli O20:K83:H26 by high resolution NMR spectroscopy

Carbohydr Res. 1994 Feb 3:253:247-56. doi: 10.1016/0008-6215(94)80069-3.

Authors

D V Whittaker¹, L A Parolis, H Parolis

Affiliation

¹ School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.

PMID: 8156551
DOI: 10.1016/0008-6215(94)80069-3

Abstract

The structure of the capsular polysaccharide produced by Escherichia coli O20:K83:H26 was investigated by one- and two-dimensional 1H and 13C NMR spectroscopy, and by glycose and methylation analysis. The capsular polysaccharide was shown to be comprised of linear pentasaccharide repeating units with the structure: [formula: see text]

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Conformation
Carbohydrate Sequence
Carbon Isotopes
Escherichia coli / metabolism*
Hydrogen
Magnetic Resonance Spectroscopy / methods
Molecular Sequence Data
Oligosaccharides / chemistry*
Oligosaccharides / isolation & purification
Polysaccharides, Bacterial / chemistry*
Polysaccharides, Bacterial / isolation & purification

Substances

Carbon Isotopes
Oligosaccharides
Polysaccharides, Bacterial
Hydrogen