Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Blood Coagul Fibrinolysis. 1993 Dec;4(6):921-6.

Urinary protein C inhibitor binding region in the B beta-chain of human fibrinogen.

Author information

  • Thrombosis Chemical Institute, Tokyo, Japan.

Abstract

The urinary protein C inhibitor (PCI) binding region in the B beta-chain of human fibrinogen was examined by ligand blotting, reverse-phase HPLC and amino acid sequencing. The B beta-chain, isolated from reduced and pyridylethylated fibrinogen, was digested with staphylococcal V8 protease to yield eight peptides consisting of 10, 12, 13, 13.5, 14, 16, 17 and 18 kDa bands and the cleaved peptides were ligand-blotted. The 12 kDa band bound to urinary PCI. Moreover, the digested B beta-chain was isolated by reverse-HPLC and the elution peak showing positive binding to urinary PCI was sequenced. The N-terminal amino acid sequence was A V S Q T, which corresponds to Ala106-Thr110 in the B beta-chain. Judging from the M(r) of this peptide (12 kDa), it comprises the region from Ala106 to Glu192-Glu210 which are partly located in the D-region. It is concluded that the urinary PCI-binding region to the B beta-chain resides in the sequence of Ala110 to Glu192-Glu210.

PMID:
8148485
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk