The relationship between current cigarette smoking and serum concentrations of vitamins C, E, and A, and of five carotenoids in human serum were examined in 91 low-income, African-American women. General linear models were used to adjust geometric mean serum concentrations of micronutrients for age, dietary and supplement intakes, total energy intake, alcohol intake, medication use, body mass index, and serum concentrations of cholesterol and triglycerides. Among smokers, serum concentrations of alpha-carotene, beta-carotene, cryptoxanthin, and lycopene averaged only 71-79% of the concentrations among nonsmokers. Mean serum concentrations of vitamins C and E and lutein/zeaxanthin were only slightly lower among smokers relative to nonsmokers, and current smokers had higher serum concentrations of vitamin A. Among current smokers, mean serum concentrations of all five carotenoids decreased with an increase in the amount smoked. The negative effect of smoking on serum concentrations of antioxidant carotenoids may pose a serious health risk in low-income populations already at higher risk for many chronic diseases.