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FEBS Lett. 1994 Mar 21;341(2-3):277-80.

Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein.

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  • 1Marine Biology Research Division, Scripps Institution of Oceanography, University of California at San Diego, La Jolla 92093.

Abstract

Expression of the cDNA for Aequorea green fluorescent protein in E. coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed with aequorin and calcium ion, emitted a greenish luminescence characteristic of the in vivo luminescence of the animal, indicating a radiationless energy transfer to the protein.

PMID:
8137953
[PubMed - indexed for MEDLINE]
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